ABSTRACT
Objective To study the expressions of Eag1 and vascular endothelial growth factor (VEGF) in colorectal cancer,and to explore the relationships between Eagl and VEGF expressions and clinicopathologic features of colorectal cancer.The correlation between Eag1 and VEGF and their clinical significance in colorectal cancer were explored.Methods A total of 73 cases of colorectal cancer tissues,25 cases of colorectal adenoma and 10 cases of normal colorectal mucosa from January 2015 to December 2016 in Hunan Provincial People's Hospital were collected.The expressions of Eag1,VEGF protein and mRNA in 73 cases of colorectal cancer tissues,25 cases of colorectal adenoma and 10 cases of normal colorectal mucosa were detected by immunohistochemistry and real-time fluorescence quantitative polymerase chain reaction (RT-qPCR).The relationships between the two factors and the clinical pathological features of patients with colorectal cancer were analyzed.Results Immunohistochemistry showed that the positive rate of Eag1 in colorectal cancer group was 86.30％ (63/73),which was significantly higher than that in colorectal adenoma group (20.00％,5/25) and normal colorectal mucosa group (10.00％,1/10),with significant differences (x2 =38.539,P ＜0.001;x2 =24.874,P ＜ 0.001).The positive rate of VEGF in colorectal cancer group was 89.04％ (65/73),which was significantly higher thah that in colorectal adenoma group (56.00％,14/25) and normal colorectal mucosa group (30.00％,3/10),with significant differences (x2 =10.980,P ＜0.001;x2 =16.911,P ＜0.001).There was a significant positive correlation between Eag1 and VEGF in colorectal cancer and colorectal adenoma tissues (c =0.580,P =0.010;c =0.669,P ＜ 0.001).The results of RT-qPCR showed that the expressions of Eag1 and VEGF mRNA in colorectal cancer group,colorectal adenoma group and normal colorectal mucosa group were 4.50 ±0.34,3.14 ±0.44;1.42 ±0.22,1.61 ±0.15 and 1.00 ±0.16,1.00 ±0.53,respectively,and the differences were statistically significant (F =15.546,P =0.014;F =5.363,P =0.025).The relative expression levels of Eag1 and VEGF mRNA in colorectal cancer group was about 4.50 times (t =31.851,P =0.003) and 3.14 times (t =6.870,P =0.014) than those in normal colorectal mucosa group.The mRNA expression levels of Eag1 and VEGF in colorectal cancer were correlated with degree of differentiation (F =840.725,P ＜ 0.001;F =102.950,P ＜ 0.001),depth of invasion (t =4.754,P ＜ 0.001;t =8.557,P ＜0.001),Duke staging (F =179.902,P ＜ 0.001;F =55.911,P ＜ 0.001) and lymph node metastasis (t =20.337,P ＜ 0.001;t =25.218,P ＜ 0.001).But they were not related to age (t =0.196,P =0.845;t =0.534,P =0.595) and sex (t =0.409,P =0.684;t =1.078,P =0.285).Conclusion Eag1 and VEGF are highly expressed in colorectal cancer tissues with positive correlation,and are closely related to the degree of differentiation,depth of invasion,Duke staging and lymph node metastasis of colorectal cancer.Both of them play important roles in the occurrence and development of colorectal cancer,which is expected to provide favorable research directions to the early diagnosis,treatment and prognosis of colorectal cancer.
ABSTRACT
Objective To study the expression and diagnostic values of long non-coding RNA(IncRNA)metastasis-associated lung adenocarcinoma transcript 1 (Malat-1),p21 and growth arrest-specific 5 (GAS5)in colorectal cancer tissue.Methods Coloreetal cancer of early stage and advanced stage(including paracareinoma tissue),precancerous lesion,benign tumor and noncancer lesion groups were collected,33 cases in each group.The levels of Malat-1,p21 and GAS5 in colorectal tissue of the 6 groups were detected by reverse transcription-polymerase chain reaction(RT-PCR).Serum carcinoembryonic antigen(CEA)and carbohydrate antigen19-9(CA19-9)were detected by ELISA.Results RT-PCR showed the levels of Malat-1,p21 and GAS5 were significantly higher in colorectal cancer of early and advanced stage and precancerous lesion than in the paracarcinoma lesion,benign tumor and non-cancer lesion(P＜0.05).Malat-1,p21 and GAS5 were the highest in the advanced stage group(P＜0.05).The levels of CEA and CA19-9 in non-cancer lesion were the least(P＜0.05).The AUC of ROC analysis operator characteristic curve(ROC)of Malat-1,p21 and GAS5 was comparable(P＞0.05).Pathological changes showed the pathological changes in colorectal cancer of early stage,advanced stage and precancerous lesion were more significant than those in the paracarcinoma lesion,benign tumor and non-cancer lesion (P＜ 0.05),which were the most significant in the advanced stage group.Conclusion The levels of Malat-1,p21 and GAS5.in colorectal cancer tissues could be good indicators for colorectal cancer diagnosis.